Isolation of a Polygalacturonase-Inhibiting Protein (PGIP) from Wheat
نویسندگان
چکیده
منابع مشابه
Characterization of the dry bean polygalacturonase-inhibiting protein (PGIP) gene family during Sclerotinia sclerotiorum (Sclerotiniaceae) infection.
Polygalacturonase-inhibiting proteins are leucine-rich repeat proteins that inhibit fungal endopolygalacturonases. The interaction of polygalacturonase-inhibiting protein with endopolygalacturonases limits the destructive potential of endopolygalacturonases and may trigger plant defense responses induced by oligogalacturonides. We examined the expression of fungal pg and plant Pvpgip genes in b...
متن کاملStructural requirements of endopolygalacturonase for the interaction with PGIP (polygalacturonase-inhibiting protein).
To invade a plant tissue, phytopathogenic fungi produce several cell wall-degrading enzymes; among them, endopolygalacturonase (PG) catalyzes the fragmentation and solubilization of homogalacturonan. Polygalacturonase-inhibiting proteins (PGIPs), found in the cell wall of many plants, counteract fungal PGs by forming specific complexes with them. We report the crystal structure at 1.73 A resolu...
متن کاملRelationships among endo-polygalacturonase, oxalate, pH, and plant polygalacturonase-inhibiting protein (PGIP) in the interaction between Sclerotinia sclerotiorum and soybean.
The necrotrophic fungal pathogen Sclerotinia sclerotiorum secretes oxalic acid and endo-polygalacturonase (endo-PG) in host plants. Oxalic acid acidifies the plant tissue to values more suitable to endo-PG activity. However, we observed that the infected soybean seedlings possessed a pH of 3.8, which is below that optimal for endo-PG activity (4.5 to 5.0). We investigated, therefore, the effect...
متن کاملThe crystal structure of polygalacturonase-inhibiting protein (PGIP), a leucine-rich repeat protein involved in plant defense.
Polygalacturonase-inhibiting proteins (PGIPs) are plant cell wall proteins that protect plants from fungal invasion. They interact with endopolygalacturonases secreted by phytopathogenic fungi, inhibit their enzymatic activity, and favor the accumulation of oligogalacturonides, which activate plant defense responses. PGIPs are members of the leucine-rich repeat (LRR) protein family that in plan...
متن کاملAn update on polygalacturonase-inhibiting protein (PGIP), a leucine-rich repeat protein that protects crop plants against pathogens
Polygalacturonase inhibiting proteins (PGIPs) are cell wall proteins that inhibit the pectin-depolymerizing activity of polygalacturonases secreted by microbial pathogens and insects. These ubiquitous inhibitors have a leucine-rich repeat structure that is strongly conserved in monocot and dicot plants. Previous reviews have summarized the importance of PGIP in plant defense and the structural ...
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ژورنال
عنوان ژورنال: Molecular Plant-Microbe Interactions®
سال: 2003
ISSN: 0894-0282,1943-7706
DOI: 10.1094/mpmi.2003.16.11.955